Culture is related to the development of our attitude. While it’s clear that companies are aware of the importance of technology when it comes to remaining competitive, digital transformation isn’t just an exercise for an organisation’s IT department. The importance of culture lies in its close association with the ways of thinking and living. For pure culture isolation, we used methods described previously for the solid-phase culture of S. glossinidius . Pure culture, in microbiology, a laboratory culture containing a single species of organism. Learn more about pure cultures in this article. A microbiological culture, or microbial culture, is a method of multiplying microbial organisms by letting them reproduce in predetermined culture medium under controlled laboratory conditions. Pure Culture Technique Culture : Act of cultivating microorganisms or the microorganisms that are cultivated Mixed culture : more than one microorganism Pure culture : containing a single species of organism. We begin by looking at the importance of culture. A pure culture is usually derived from a mixed culture (one containing many species) by transferring a small sample into new, sterile growth medium. It’s also, crucially, very much a people journey. Without high-quality media, the possibility of achieving accurate, reproducible, and repeatable microbiological test results is reduced. Differences in cultures have led to a diversity in the people from different parts of the world. Our moral values represent our culture. Culture media is of fundamental importance for most microbiological tests: to obtain pure cultures, to grow and count microbial cells, and to cultivate and select microorganisms. Briefly, 100 to 200 μl of liquid from the 5-day-old pupal culture (described above) was streaked onto sterile agar plates containing MM medium supplemented with 0.7% agar.